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Place the preposition since before words or phrases denoting a point in time: since Monday, since yesterday, since eight o'clock, since Christmas. When we use since, the verb is usually in the present perfect tense, but it may be in the past perfect: I was glad to see Tom. I hadn't seen him since last Christmas.
From can also denote a point in time, but it must be followed by to or till: He works from eight o'clock till one o'clock without a break.
We use in to describe the physical location of something as part of a larger thing or place. We use at when we're talking about an address, a public place or building (a bus stop, the Post Office, the library etc.) and cases in which the location is irrelevant but what we do there is what matters (school, the dentist, dance class etc.)
When speaking of a period of time in the future, use in, and not after. Here in means after the end of.